N benthamiana

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Nicotiana benthamiana , colloquially known as benth or benthi , is a species of Nicotiana indigenous to Australia. It is a close relative of tobacco. A synonym for this species is Nicotiana suaveolens var. This was transferred to Nicotiana benthamiana by Karel Domin in Bibliotheca Botanica , honoring the original author in the specific epithet. The plant was used by people of Australia as a stimulant, containing nicotine and other alkaloids , before the introduction of commercial tobacco N. Indigenous names for it include tjuntiwari and muntju.

N benthamiana

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. An Author Correction to this article was published on 05 January To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions. Despite the loss of five chromosomes from the ancestral tetraploid, expansion of intergenic regions, widespread segmental allopolyploidy, advanced diploidization and evidence of recent bursts of Copia pseudovirus Copia mobility not seen in other Nicotiana genomes, the two subgenomes of N. LAB and QLD have many genetic, metabolic and phenotypic differences, including disparate RNA interference responses, but are highly interfertile and amenable to genome editing and both transient and stable transformation. Like most Solanaceae, it has a basic chromosome number of 12, with haploid DNA content ranging from 1. Section Suaveolentes nicely smelling includes N. The diploid ancestors of N.

An accessory protease inhibitor to increase the yield and quality of a tumour-targeting mAb in Nicotiana benthamiana leaves. Email address Sign up. Protection n benthamiana recombinant mammalian antibodies from development-dependent proteolysis in leaves of Nicotiana benthamiana.

Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, mature, and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host Nicotiana benthamiana as a model to map the accumulation profile of influenza virus hemagglutinin H1, a clinically promising vaccine antigen, at the whole plant scale. Greenhouse-grown plants submitted to different light regimes, submitted to apical bud pruning, or treated with the axillary growth-promoting cytokinin 6-benzylaminopurine were vacuum-infiltrated with agrobacteria harboring a DNA sequence for H1 and allowed to express the viral antigen for 7 days in growth chamber under similar environmental conditions. Our data highlight the importance of young leaves on H1 yield per plant, unlike older leaves which account for a significant part of the plant biomass but contribute little to total antigen titer. These findings underline the relevance of both considering main stem leaves and axillary stem leaves while modeling heterologous protein production in N. They also demonstrate the potential of exogenously applied growth-promoting hormones to modulate host plant architecture for improvement of protein yields.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. An Author Correction to this article was published on 05 January To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions. Despite the loss of five chromosomes from the ancestral tetraploid, expansion of intergenic regions, widespread segmental allopolyploidy, advanced diploidization and evidence of recent bursts of Copia pseudovirus Copia mobility not seen in other Nicotiana genomes, the two subgenomes of N. LAB and QLD have many genetic, metabolic and phenotypic differences, including disparate RNA interference responses, but are highly interfertile and amenable to genome editing and both transient and stable transformation.

N benthamiana

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Nicotiana benthamiana is a workhorse for fundamental research and biotechnology.

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Medicago Inc. Synteny and orthogroup analyses with other Solanaceae Genome sequence comparisons were performed using D-Genies Cabanettes and Klopp Highly suppressed genes in both subgenomes were assessed. A related question is whether such eventual effects of cultural practices can be harnessed to generate protein yield gains on a whole plant basis. To investigate this further, a cluster analysis was made using the proteomes predicted from our LAB assembly and the available scaffold assemblies of N. Solanaceae—a model for linking genomics with biodiversity. Distances between genes are indicated in Mb. Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in Nicotiana benthamiana. Faye, L. Section Suaveolentes nicely smelling includes N. Whole-genome bisulfite sequencing Whole-genome bisulfite sequencing samples were prepared with genomic DNA extracted from the same tissues used for chromatin immunoprecipitation sequencing.

Nicotiana benthamiana is widely used as a model plant for dicotyledonous angiosperms. In fact, the strains used in research are highly susceptible to a wide range of viruses.

Wang, Y. Supplementary Table 7C Characteristics and sources of genomes used for comparative analyses. The genome and metabolome of the tobacco tree, Nicotiana glauca : a potential renewable feedstock for the bioeconomy. B A phylogenetic tree constructed among the six plant species. Shao, M. The establishment of Suaveolentes section including N. Jassbi, A. Subsequently, simple sequence repeat SSR markers identified in N. You are using a browser version with limited support for CSS. Gene prediction and annotation A total of 1. This, along with a harvested biomass for S3 much greater than the biomass harvested for S1 and S2, prevented the onset of a leaf age-related decline of total H1 yield in S production units such as that observed in the P units Figures 2B , C.