Smai cut site

Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER

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Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation This will prevent the Sma I ends of the vector from religating. This will usually go into solution if you warm the tube to 37C and then vortex it briefly. You may wish to prepare your own buffer. To prepare 10 mL of buffer, weigh 2.

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Enzymes and Inhibitors. Restriction Enzymes. Catalog number: ER Related applications: Restriction Enzyme Cloning. Technical Support Customer Service. Catalog Number.

Smai cut site

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Enter your username and we'll send a link to reset your password. So my situation now is I'm trying to cut a plasmid bp with smaI. Frequently asked questions FAQs. Name This field is required. Password Password doesn't meet requirements. You have 1 remaining attempt. In addition, the universal Tango buffer is provided for convenience in double digestions. After that, you will need to contact Customer Service to unlock your account. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. Catalog Number. Confirm Password Passwords don't match. You have successfully reset your password. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Learn more. We are excited to announce that all reaction buffers are now BSA-free. Find more details at www.

Preference Cookies We use these cookies to remember your settings and preferences. Hope you guys can shed some light Confirm New Password Passwords don't match. Hi all, I juz started with cloning in pUC using SmaI to restrict digest for blunt ends and when i ran the gel got a linear plasmid but dint do dephosphorylation BRL Technical Bulletin You may wish to prepare your own buffer. Add to Helix This product is available through the Promega Helix onsite stocking program. V, V, DV Ethidium Bromide Solution, Molecular Grade Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. Password reset is required. We use these cookies to collect information about how you interact with our services and to help us measure and improve them. To prepare 10 mL of buffer, weigh 2. A verified email address is required to access the full functionality of your Promega. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.