Spectra viewer

The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location, spectra viewer.

Navigation Menu. AAT Bioquest. Cart 0. Sign In. Add a spectrum to begin.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument.

A menu will appear below the graph display with common generic lasers displayed on the left. It streamlines experiment design spectra viewer helps to understand which fluorochromes might cause problems, saving troubleshooting time, spectra viewer. If the desired excitation source is known, click to select.

.

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment.

Spectra viewer

Navigation Menu. AAT Bioquest. Cart 0. Sign In. This can be useful for rotating through several overlapping spectra x. Add a spectrum to begin. Compound Name. How to use this tool AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats.

Water temperature fort walton beach florida

To select a known filter, select one or more options on the left menu. The user may move this excitation source by hovering over the representative vertical line until the cursor changes shape to a symmetrical horizontal arrow; once the cursor has changed shape, left-click and hold while dragging the line to a desired place along the horizontal axis. To instead share a link to the graph s page, click "Shareable Link" to copy the url to the clipboard. Spectra Viewer and Experiment Design. If the "Custom" row is selected, a popup box will appear and prompt the user to enter the center wavelength. The excitation source will now appear on the graph display as a thin vertical line in a color associated with its location on the spectrum. To search for an appropriate filter, use the right-side menu section to search by part type, manufacturer, or instrument. The display will adjust to the desired settings. This may be a convenient setting for users employing low-contrast screens. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters. To continue, enter the center wavelength value as a number and click "OK". Click "Disable Intensity Prediction" to return to the default visual display. To search for dyes by feature, use the section on the right and enter in the desired values, then press the ENTER key. These percentages are automatically calculated for each compound currently on the graph.

.

For technical assistance on using this web application, please contact websupport aatbio. If the desired excitation source is known, click to select. Open Filter Coverage to show a spillover table which reports the percentage of the emission curve that is contained in each selected filter. How to use this tool AAT Bioquest's interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats. The user may then "paste" the link into an email or other form of digital communication by either using the right mouse button or clicking the "CTRL" and "v" keys simultaneously. To search for dyes by feature, use the section on the right and enter in the desired values, then press the ENTER key. The excitation source will now appear on the graph display as a thin vertical line in a color associated with its location on the spectrum. The user may move this excitation source by hovering over the representative vertical line until the cursor changes shape to a symmetrical horizontal arrow; once the cursor has changed shape, left-click and hold while dragging the line to a desired place along the horizontal axis. The display will adjust to the desired settings. A menu will appear below the graph display with common generic lasers displayed on the left. Revvity Sites Globally Select your location. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument.