Superscript iii one step rt pcr system

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Superscript iii one step rt pcr system

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Superscript iii one step rt pcr system

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Hotplates and Stirrers. Real-time polymerase chain reaction. The site is secure. Laboratory Benches and Tables. The sensitivity of one-step versus two-step methods was compared using the cycle threshold values. Copy Download. Reverse Transcriptase. Typically, scientists choose one-step or two-step methods based on these factors. Cell Culture Media. Thermo Fisher Financial Services. Reaction efficiencies were determined by generating standard curves using total RNA isolated from human skeletal muscle and brain. SuperScript III. A: Real-time fluorescence using fold dilutions of total RNA from skeletal muscle run in triplicate.

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Respiratory Protection. Reagent Type. The largest CV for two-step was 1. Help Desk. Production Applications. The same threshold value was used in order to compare the cycle threshold values of the methods. Likewise, factors such as the tissue type and gene expression level also did not significantly alter the reaction efficiencies or R 2 values during the real-time runs. The RNA from the original sample must be initially aliquoted for archival storage and future testing. Biotechniques ; 29 , , Laboratory Benches and Tables. Ribonuclease H Activity. ULS Inventory Manager. While several of these factors may play a role in the difference in sensitivity, our findings provide support for the use of the one-step method for increased sensitivity of detection of certain genes such as PolR2A. Detection Method. A 5-cycle difference indicates a fold change in detection.