Cellosaurus

We have developed an online database describing the known cell lines from Coleoptera, Iconic blazers, Hemiptera, Hymenoptera, and Lepidoptera that were developed from agricultural pests. Cell line information has been primarily obtained from previous compilations of insect cell lines, cellosaurus. Here, we report on a new database of cellosaurus cell lines, which covers cell lines from 86 species. We provide the designation, tissue and species of origin, cellosaurus line cellosaurus, unique characteristics, its use in various applications, publications, and patents, and, when known, insect virus susceptibility.

Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research. To prevent it, researchers are expected to frequently test the authenticity of the cell lines they are working on. STR profiling was selected as the international reference method to perform cell line authentication. While the experimental protocols and manipulations for generating a STR profile are well described, the available tools and workflows to analyze such data are lacking. The Cellosaurus knowledge resource aimed to improve the situation by compiling all the publicly available STR profiles from the literature and other databases.

Cellosaurus

This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful new resources with the scientific community — particularly ones that help address ongoing problems, like cells lines. A study in Science found that approximately one-third of cell lines used in scientific research are misidentified! Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus , a growing cell line information database describing nearly , cell lines. Cellosaurus seeks to identify and describe all cell lines used in research, using four methods to search out cell lines:. Entries include accession number, species, and assigned category cancerous, hybridoma, etc. STR profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email for cell lines profiled in the lab. You can browse entries by cell line groups , such as their vaccine production , SARS-CoV-2 research , or adenovirus packaging cell lines dataset collections. Importantly, Cellosaurus also identifies problematic cell lines: ones either suspected to be contaminate or easily contaminated, as well as commonly misidentified lines. Browsing this feature to know if your lines may be especially vulnerable can be very informative. Spoiler alert: HeLa is the most common contaminator line. For those wanting to access Cellosaurus programatically, their API allows fine grained query and retrieval of all the information in the resource. This is a labor-intensive project; scripts can be used in some cases, but much of this work has required manually going through the pdfs of the 26, publications cited.

Stay connected! Using the data provided by the cellosaurus, Cellosaurus was able to both update their database and sent the updated information to the cell line distributor, cellosaurus. A success all around!

Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely. The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. Other types of information include standardised disease terminology for cancer or genetic disorder cell lines , the transformant used to immortalise a cell line, transfected or knocked-out genes, microsatellite instability , doubling time , gender and age of donor patient or animal , important sequence variations, web links, publication references and cross-references to close to different databases, ontologies, cell collections and other relevant resources. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables. For human as well as some dog cell lines, it provides short tandem repeat STR profile information.

Federal government websites often end in. The site is secure. The Cellosaurus is a knowledge resource on cell lines. It aims to describe all cell lines used in biomedical research. Its scope encompasses both vertebrates and invertebrates.

Cellosaurus

Cellosaurus is an online knowledge base on cell lines , which attempts to document all cell lines used in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely. The Cellosaurus provides a wealth of manually curated information; for each cell line it lists a recommended name, synonyms and the species of origin. Other types of information include standardised disease terminology for cancer or genetic disorder cell lines , the transformant used to immortalise a cell line, transfected or knocked-out genes, microsatellite instability , doubling time , gender and age of donor patient or animal , important sequence variations, web links, publication references and cross-references to close to different databases, ontologies, cell collections and other relevant resources. Since many cell lines used in research have been misidentified or contaminated , the Cellosaurus keeps track of problematic cell lines, including all those listed in the International Cell Line Authentication Committee ICLAC [8] tables. For human as well as some dog cell lines, it provides short tandem repeat STR profile information.

Successfully synonym

Abstract The Cellosaurus is a knowledge resource on cell lines. Sharing science just got easier It may require cleanup to comply with Wikipedia's content policies, particularly neutral point of view. S2CID Retrieved Download as PDF Printable version. Tools Tools. Categories : Molecular biology Biological databases Cell biology Cell culture. The Cellosaurus is a knowledge resource on cell lines. Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus , a growing cell line information database describing nearly , cell lines. Publications A database of crop pest cell lines. If you have cell lines without a certified origin you know, that split you got from the graduate student down the hall who got it from some technician downstairs who got it from who knows where , now is as good a time as ever to find out the true identity of the line? Here, we report on a new database of insect cell lines, which covers cell lines from 86 species. To prevent it, researchers are expected to frequently test the authenticity of the cell lines they are working on. Since July , cell lines in the Cellosaurus are represented as items in Wikidata.

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For instance, recently Cellosaurus received a report that HT, which was described as a colon cancer cell line, is actually a rectal cancer cell line. Browsing this feature to know if your lines may be especially vulnerable can be very informative. As a result, it grew to become the largest database in terms of human STR profiles, with 6, distinct cell lines having an associated STR profile release July 31, Science ; — Database Profile Cellosaurus. We store and distribute high-quality plasmids from your colleagues. Stay connected! Since July , cell lines in the Cellosaurus are represented as items in Wikidata. Archived PDF from the original on To prevent it, researchers are expected to frequently test the authenticity of the cell lines they are working on. Branches of biology. Word cloud. It aims to help researchers in the process of cell line authentication by providing numerous functionalities. Abstract Despite an increased awareness of the problematic of cell line cross-contamination and misidentification, it remains nowadays a major source of erroneous experimental results in biomedical research. Its scope includes immortalised cell lines , naturally immortal cell lines example: embryonic stem cells and finite life cell lines when those are distributed and used widely.